主要研究業績 (2005以後)


Ohmae,E., Hamajima,Y., Nagae,T., Watanabe,N., and Kato,C. "Similar structural stabilities of 3-isopropylmalate dihydrogenases from the obligatory piezophlic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1" Biochim. Biophys. Acta, in press (2018).

Saito,M., Hiratoko,S., Fukuba,I., Tate,S., and Matsuoka,H., "Use of a right triable chip and its engraved shape as a transferrable x-y coordiante system from light microscopy to electron microscopy" Electrochemistry, 86, 6-9 (2018).

Umehara,K., Hoshikawa,M., Tocho,N., and Tate,S., "Substrate binding switches the conformation at the lychpin site in the substrate-binding domain of human Hsp70 to enable allosteric interdomain communication" Molecules, 23, 528 (2018).

Tate,S., "Protein structure and dynamics determination by residual anisotropic spin interactions" Experimental Approaches of NMR Spectroscopy (The Nuclear Magnetic Resonance Society of Japan Ed.), Springer (2018).


Wang,J., Kawasaki,R., Uewaki,J., Rashid,A.U.R., Tochio,N., and Tate,S., "Dynamic allostery modulates catalytic activity by modifying hydrogen bonding netwrok in the catalytic site of human Pin1" Molecules, 22, 992 (2017).

Yoshimura,Y., Oktaviani,N.A., Yonezawa,K., Kamikubo,H., and Mulder,F.A.A., "Unambiguous determination of protein arginine ionization states in solution by NMR spectroscopy" Angew. Chem. Int. Ed., 56, 239-242 (2017).

Nakamura,A., Tochio,N., Fuijoka,S., Ito,S., Kigawa,T., Shimada,Y., Matuoka,M., Yoshida,S., Kinoshita,T., Asami,T., Seto,H., "Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice" PLoS One, 12, e0174015 (2017).

Makino,A., Abe,M., Ishitsuka,R., Murata,M., Kishimoto,T., Sakai,S., Hullin-Matuda,F., Shimada,Y., Inaba,T., Miyatake,H., Tanaka,H., Kurahashi,A., Pack,C.G., Ksai,R.S., Kubo,S., Schieber,N.L., Dohmae,N., Tochio,N., Hagiwara,K., Sasaki,Y., Aida,Y., Fujimori,F., Kigawa,T., Nishibori,K., Parton,R.G., Kusumi,A., Sako,Y., Anderluh,G., Yamashita,M., Kobayashi,T., Greimel,P., and Kobayashi,T., "A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C" FASEB J., 31, 1301-1322 (2017).

Eguchi,Y., Okajima,T., Tochio,N., Inukai,Y., Shimizu,R., Ueda,S., Shinya,S., Kigawa,T., Fukamizo,T., Igarashi,M., and Utsumi,R., "Angucycline antibiotic waldiomycin recognizes common structural motif conserved in bacterial hitidine kinases" The Journal of Antibiotics, 70, 251-258 (2017).

Kuwasako,K., Nameki,N., Tsuda,K., Takahashi,M., Sato,A., Tochio,N., Inoue,M., Terada,T., Kigawa,T., Kobayashi,N., Shirouzu,M., Ito,T., Sakamoto,T., Wakamatsu,K., Guntert,P., TAkahashi,S., Ykoyama,S., and Muto,Y., "Solution structure of the first RNA recognition motif domain of human splicesomal protein SF3b49 and its mode of interaction with a SF3b145 fragment" Protein Sci., 26, 280-291 (2017).

Hiraishi,N., Maruno,T., Tochio,N., Sono,R., Otuki,M., Takatsuka,T., Tagami,J., and Kobayashi,Y., "Hesperidin interaction to collagen detected by physico-chemical techniques" Dental materials, 33, 33-42 (2017).

Oktaviani,N.A., Pool,T.J., Yoshimura,Y., Kamikubo,H., Scheek,R.M., Kataoka,M., Mulder,F.A.A., "Active-site Pka Determination for photoactive yellow protein rationalizes slow ground-state recovery" Biophy. J., 112, 2109-2116 (2017).

Miyashita,Y., Ohmae,E., Ikura,T., Nakasone,K., and Katayanagi,K., "Halophilic mechanism of the enzymatic function of moderately halophilic dihydropholate reductase from Haloarcula japonica strain TR-1" Extremophiles, 21, 591-602 (2017).

楯 真一 "TALEタンパク質の超らせん運動とDNA配列認識"生物物理 57, 127-130 (2017).

大前英司 "高圧処理による酵素の活性化と不活性化"食品と容器 58,404-410 (2017).


Tochio,N., Umehara,K., Uewaki,J., Flechsig,H., Kondo,M., Dewa,T., Sakuma,T., Yamamoto,T., Saitoh,T., Togashi,Y., and Tate,S. "Non-RVD mutations that enahcne the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy"Scientific Reports., 6, 37887 (2016).

Narayana,S.P., Maeno,A., Wada,Y., Tate,S., and Akasaka,K. "Sequential backbone resonance assignments of the E.coli dihydrofolate reductase Gly67Val mutant:folate complex" Biomol. NMR Assign., 10, 125-129 (2016).

Ohame,E., Miyashita,Y., and Kato,C. "Functional, structural, and thermodynamic characteristics of enzymes from deep-sea microorganisms" in Microbial Catalyst 2nd Ed. (Eds. Adbel-Aziz, Garg,N. Aeron,A., Gupta,V.K., Jha,C.K., and Nayak,S.C.) (2016).

Hamajima,Y., Nage,T., Watanabe,N., Ohmae,E., Kato-Yamada,Y., and Kato,C. "Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution."Extremophiles, 20, 177-186 (2016).

大前英司,宮下由利奈,加藤千明 "酵素の構造安定性と機能におけるキャビティーと水和の役割-新海微生物由来酵素からの知見-"熱測定 43,59-65 (2016).


Wang,J., Tochio,N., Kawasaki,R., Tamari,Y., Xu,N., Uewaki,J., Utsunomiya-Tate,N., and Tate,S. "Allosteric breakage of the hydrogen bond within the dual-histidine motif in the active site of human Pin1 PPIase", Biochemistry, 54, 5242-5253 (2015).

Takami,T., Ojiro,Y., Ogawa,S., Takakuwa,Y., Ogawa,Y., Saito,M., Matsuoka,H., and Tate,S. "Coating the outer surface of glass nanopipette with chlorobenzen-terminated polysiloxane" e-J.SurfSci. Nanotech. 13, 79-84 (2015).

Ono,Y., Miyashita,M., Ono,Y., Okazaki,H., Watanabe,S., Tochio,N., Kigawa,T., Nishimura,C. "Comparison of residual alpha- and beta-structures between two intrinsically disordered proteins by using NMR", Biochim. Biophys. Acta. 1854, 229-238 (2015).

Hiraishi,N., Tochio,N., Kigawa,T., Otsuki,M., and Tagami.J., "Molecular level evaluation on HEMA interaction with a collagen model", Dent. Mater. 31, 88-93 (2015).

Miyashita,Y., Ohmae,E., Nakasone, and Katayanagi,K. "Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarula japonica strain TR-1", Extremophiles, 19, 479-493 (2015).

Ohmae,E., Tanaka,S., Miyashita,Y., Katayanagi,K., and Matsuok,K. "Vacuum-ultraviolet circular dichroism spectra of Escherichia coli dihydrofolate reductase and its mutaants: Contributions of phenylalanine and tyrosine side chains and exciton coupling of two tryptophan side chains.", J.Phys.Chem., B119, 13002-13008 (2015).

Ohmae,E., Gekko,K., and Kato,C. "Environmental adaptation of dihydrofolate reductase from deep-sea bacteria."in High pressure bioscience-Basic concepts, applications and frontiers (Eds. Akasaka,K. and Matsuki,H.),Springer (2015).

大前英司,宮下由利奈,月向邦彦,加藤千明 "海微生物由来ジヒドロ葉酸還元酵素の高圧力環境適応機構" 高圧バイオサイエンスとバイオテクノロジー(野村一樹,藤沢哲朗,岩橋均 編集) pp130-140, 三恵社 (2015).


Xu,N., Tochio,N., Wang,J., Tamari,Y., Uewaki,J., Utsunomiya-Tate,N., Igarashi,K., Shiraki,T., Kobayashi,N., and Tate,S. "The C113D mutation in human Pin1 causes allosteric structural changes in the phosphate binding pocket of the PPIase domain through the tug of war in the dual-histidine motif", Biochemistry, 53, 5568-5578 (2014).

Okazaki,H., Kaneko,C., Hirahara,M., Watanabe,S., Tochio,N., Kigawa,T., and Nishimura,C. "Long-range effects of tag sequence on marginally stabilized structure in HIV-1 p24 capsid protein monitored using NMR", Biochim. Biophys. Acta., 1844, 1638-1647 (2014).

Voet,A.R.D., Ito,A., Hirohama,M., Matuoka,S., Tochio,N., Kigawa,T, Yoshida,M., and Zhang, K.Y.J. "Discovery of small molecule inhibitors targetting the USMO-SIM interaction using a protein interface consensus approach" MedChemComm., 5, 783-786 (2014).

Hiraishi,N., Tochio,N., Kigawa,T., Otsuki,M., and Tagami,J. "Role of 2-hydroxyethyl methancrylate in the interaction of dental monomers with collagen studied by saturation transfer difference NMR", J.Dent. 42, 484-489 (2014).

Ohori,Y., Okszaki,H., Watanabe,S., Tochio,N., Arai,M., Kigawa,T., and Nishimura,C. "Flexible and rigid structures in HIV-1 p17 matrix protein monitored by relaxation and amide proton exchange with NMR", Bioshim. Biophys., Acta, 1844, 520-526 (2014).

Deng,X.L., Takami,T., Son,J.W., Kang,E.J., Kawai,T., Park,B.H., "Effect of concentration gradient on ionic current rectification in polyethyleneimine modified glass nano-pipettes.", Sci. Rep. 4, 4005 (2014).

Takami,T., "Manipulation of organic molecules in ambient condition studied by scanning tunneling microscopy", e-J. Surf. Sci. Nanotech., 12, 157-164 (2014).

Takami,T., Kawai,T., Park,B.H., "Nanopipette exploring nanoworld", Nano Convergence, 1, 17 (2014).

Kang,E.J., Takami,T., Deng,X.L., Son,J.W., Kawai,T., Park,B.H., "Improved ion-selective detection method using nano-pipette with poly(vinylchloride)-based membrane", J.Chem.Phys. B 118, 5130-5134 (2014).


Wang,J., Tochio,N., Takeuchi,A., Uewaki,J., Kobayashi,N., and Tate,S. "Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA" BBRC, 441, 701-706 (2013).

Ohame,E., Miyashita,Y., Tate,S., Gekko,K., Kitazawa,S., Kitahara,R., and Kuwajima,K., "Solvent environment significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: comparison of wild-type protein and active-site mutant D27E", BBA - proteins and proteomics, 1834, 2782-2794 (2013).

Okazaki,H., Ohri,Y., Komoto,M., Lee,Y.H., Goto,Y., Tochio,N., Nishimura,C. "Remaining structures at the N- and C-terminal regions of alpha- synuclein accurately elucidated by amide-proton exchange NMR with fitting", FEBS Lett., 587, 3709-3714 (2013).

Harada,R., Tochio,N., Kigawa,T., Sugita,Y., Feig,M., "Reduced native state stability in crowded cellular environment due to protein-protein interactions", J.Am.Chem.Soc. 135, 3696-3701 (2013).

Hiraishi,N., Tochio,N., Kigawa,T., Otsuki,M., Tagami,J., "Monomer-collagen interactions studied by saturation transfer difference NMR", J.Dent.Res. 92, 284-288 (2013).

Shimahara,H., Hirano,T., Ohya,K., Matsudas,S., Seeram,Sailaja, and Tate,S. "Nucleosome structural changes induced by binding of non-hisotne chromosomal proteins HMGN1 and HMGN2" FEBS Open Bio 3, 184-191 (2013).

Uewaki,J., Kamikubo,H., Kurita,J., Hiroguchi,N., Moriuchi,H., Yoshida,M., Kataoka,M., Utsunomiya-Tate,N., and Tate,S. "Preferential domain orientation of HMGB2 determiend by the weak intramolecular interactions mediated by the interdomain linker" Chemical Physics, 419, 212-223 (2013).

Hashimoto,M., Kodera,N., Tsunaka,Y., Oda,M., Tanimoto,M., Ando,T., Morikawa,K., and Tate,S. "Phosphorylation-coupled intramolecular dynamics of unstructured regions in chromain remodeler FACT" Biophys.J. 104, 2222-2234 (2013).

Ohmae,E., Matsuo,K., and Gekko,K., "Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: insight into the contribution of tryptophan residues" Chem. Phys. Lett. (2013) in press.

楯 真一 「NMRによる蛋白質の動的構造解析」 生化学 85, 638-645 (2013).

大前英司 「タンパク質の構造安定性と機能における水和の役割−深海微生物由来酵素の研究からわかること」 高圧力の科学 23, 13-20 (2013)


Nakano,S., Sugihiara,M., Yamada,R., Ktayanagi,K., and Tate,S. "Structural implication for the impaired binding of W150A mutant LOX-1 to oxidized low density lipoprotein, OxLDL. Biochimica et Biophysica Acta, 1824, 739-749 (2012).

Matsuo,K., Sakurada,Y., Tate,S., Namatame,H., Taniguchi,M. and Gekko,K. "Secondary-structure analysis of alchol-denatured proteins by a vacuum-ultraviolet circular dichroism spectroscopy" PROTEINS, 80, 281-293 (2012)

Ohmae,E., Murakami,C., Tate,S., Gekko,K, Hata,K, Akasaka,K., and Kato,C. "Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli. Biochimica et Biophysica Acta, 1824, 511-519 (2012)

Nakano,S., Takhashi,M., Sakamoto,A., Morikawa,H., and Katayanagi,K., "Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high resolution structure", Protein Sci. 21, 283-395 (2012).

Nakano,S., Takahashi,M., Sakamoto,A., Morikawa,H., and Katayanagki,K., "The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystasl structures and ultraviolet-visible microspectroscopy", PROTEINS, 80, 2035-2045 (2012)

Nakano,S., Takahashi,M., Sakamoto, A., Morikawa,H., and Katayanagi,K., "X-ray crystal structure of a mutant assimilatory nitride reductase that shows sulfite reductase-like activity", Chemistry & Biodiversity, in press (2012).


Tate,S., Imada, A., and Hiroguchi,N. "Comprementary use of NMR to X-ray crystallography for the analysis of protein morphological change in solution" (Chapter 18), pp409-436 in "X-ray Crystallography" InTech, Rijeka, Croatia (invited review) (2011)

Mizuno,S., Amida,H., Kobayashi,N., Aizawa,S., and Tate,S. "The NMR structure of FliK, the trigger for the switch of substrate specificty in the flagellar type III secretion appratus" J.Mol.Biol. 409, 558-573 (2011)

Ohki,I., Amida,H., Yamada,R., Sugihara,M., Ishigaki,T. and Tate,S. "Surface plasmon resonance study on functional significance of clustered organization of lectin-like oxidized LDL receptor 1 (LOX-1)" Biochimica et Biophysica Acta 1814, 345-354 (2011)

Murakami,C., Ohmae,E., Tate,S., Gekko,K., Nakasone,K., and Kato,C. "Comparative study on dihydrofolate reductases from Schwanella species living in deep-sea and ambient atmospheric-pressure environments" Extremophiles, 15, 165-175 (2011)

Nakano,S., Takahashi,M., Sakamoto,A., Morikawa,H. and Katayanagi,K. "The initial N-O bond cleavage mechanism of nitrite in assimilatory-nitrite-reductase from tabacum: effective utilization of X-ray structures and UV-Vis microspectroscopy" J.Biol.Inorg.Chem. 16 (suppl.1) S55 (2011)

楯 真一 「NMRで観測する超高分子量タンパク質の分子形態」 生物物理, 51, 84-87 (2011)

楯 真一 「蛋白質の分子形態変化の観測」 (章著) 広がるNMRの世界(朝倉哲朗 編) コロナ社 (2011)


Horiuchi,Y., Ohmae,E., Tate,S. and Gekko,K. "Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase reveased by deletion mutants" Biochimica et Biophysica Acta 1804, 846-855 (2010)

Murakami,C., Ohmae,E., Tate,S., Gekko,K., Nakasone,K., and Kato,C. "Cloning and characterization of dihydrofoalte reductases from deep-sea bacteria" J.Biochem. (Tokyo) 174, 591-599 (2010)

Fujimoto,Y., Shiraki,T., Horiuchi,Y., Waku,T., Shigenaga,A., Otaka,A., Ikura,T., Igarashi,K., Aimoto,S., Tate,S., and Morikawa,K. "Prorine cis/trans isomerse Pin1 regulates peroxisome proliferator-activated recetpro g activity through the direct binding to the AF-1 domain" J.Biol.Chem. 285, 3126-3132 (2010)

楯 真一 「動脈硬化初期病巣の選択的磁気造影(MRI)のための基礎研究技術開発」 医学応用財団報告書 28, 171-178 (2010)


Matsuo,K., Watanabe,H., Tate,S., Tachibana,H., and Gekko,K. "Comprehensive secondary-structure analysis of disulfide variants of lysozyme by synchrotron-radiation vacuum-ultraviolet" PROTEINS: Structure, Function, and Bioinformatics 77, 191-201 (2009)

Tsunaka,Y., Toga,J., Yamaguchi,H., Tate,S., Hirose,S. and Morikawa,K. "Phosphorylated intrinsically disordered region of FACT masks nucleosomal DNA binding elements" J.Biol.Chem. 284, 24610-24621 (2009)

Gekko,K., Araga,M., Kamiyam,T., Ohmae,E., and Akasaka,K., "Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities" Biophysical Chemistry 144, 67-71 (2009)


Tate,S. "Anisotropic nuclear spin interactions for the morphology analysis of proteins in solution NMR spectroscopy" Anal. Sci. 24, 39-49 (2008).

Ohmae,E., Tatuta,M., Abe,F., Kato,C., Tanaka,N., Kunugi,S., and Gekko,K. "Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase" Biochim. Biophys. Acta - proteins and proeomics 1784, 1115-1121 (2008)

Kato,C., Sato,T., Abe,F., Ohmae,E., Tamagai,H., Nakanone,K., Siddiqui,K.S., and Thomas,T. "Protein adaptation to high-pressure environments" in "Protein Adaptation in Extremophiles" (Ed. Thomas, T. and Siddiqui,K.S.) pp 167-191 Nova Science Publiser (2008)

楯 真一 「タンパク質構造の揺らぎを捉える新しいNMR構造解析技術」 化学と工業 61, 234-235 (2008)

大前英司,村上千穂,楯 真一,月向邦彦,加藤千明 「蛋白質に対する圧力の効果」 高圧バイオサイエンスとバイオテクノロジー 2, 38-44 (2008) 

加藤千明,佐藤孝子,阿部文快,大前英司,為我井秀行,仲宗根薫 「高圧下への蛋白質の適応」 高圧バイオサイエンスとバイオテクノロジー 2, 45-53 (2008) 


Shimahara,H., Yoshida,T., Shibata,Y., Shimizu,M., Kyogoku,Y., Sakiyama,F. Nakazawa,T., Tate,S., Ohki,S., Kato,T., Moriyama,H., Kishida,K., Tano,Y., Ohkubo,T., and Kobayashi,Y. "Tautomerism of histidine 64 associated with proton transfer in catalysis of carbonic anhydrase" J.Biol.Chem. 282, 9649-9656 (2007)

Tate,S. "Oxidized low-density lipoprotein receptor, LOX-1, on the endothelial cell - the receptor structure and functions of LOX-1 in antherogeneis" J.Biol. Macromol. 7, 11-22 (2007)

Ishigaki,T., Ohki,I., Utsunomiya-Tate,N., and Tate,S. "Chimeric structural stabilities in the coiled-coil structure of the NECK domain in human lectin-like oxidiazed low-density lipoprotein receptor 1 (LOX-1)" J.Biochem. (Tokyo) 141, 855-866 (2007)

Ohmae,E. Murakami,C., Gekko,K. and Kato,C. "Pressure effects on enzyme function" J.Biol. Macromol. 7, 23-29 (2007)


Tate,S. "Structure and mode of ligand recognition of the oxidized LDL recetpor" in "Functional and Structural biology on the lipo-network" Research Signpost, Kerala, India, 179-198 (2006)

Takeshima,H., Suetake,I., Shimahara,H., Ura,K., Tate,S., and Tajima,S. "Distinct DNA methylation activity of Dnmt3a and Dnmt3b towards naked and nucleosomal DNA" J.Biochem. (Tokyo) 139, 503-515 (2006)

Functional and Structural Biology on the Lipo-network (2006) Editor-in-Chief: Kousuke Morikawa, Co-editor: Shin-ichi Tate

楯 真一 「核スピン系のダイナミクス」 化学実験講座8 NMR・ESR (1. NMRの基礎 1.5節) pp40-69 (2006)

楯 真一 「異種核相関」 化学実験講座8 NMR・ESR (3. 溶液における各種二次元NMR法 3.2節) pp162-192 (2006)


Okuwaki,M., Kato,K., Shimahara,H., Tate,S., and Nagata,K. "Assembly and disassembly of nucleosome core particles containing histone variants by human nuclesome assembly protein-I" Mol.Cell.Biol. 25, 10639-10651 (2005)

Ohki,I., Ishigaki,T., Oyama,T., Matsunaga,S., Xie,Q., Ohnishi-Kameyama,M. Murata,T., Machida,S., Morikawa,K., and Tate,S. "Crystal structure of human lectin-like oxidized low-density lipoprotein receptor (LOX-1) ligand-binding domain and identification of its binding surface to ligands" Structure 13, 905-917 (2005)

Ishigaki,T., Ohki,I., Oyama,T., Machida,S., Morikawa,K., and Tate,S. "Purification, crystallization, and preliminary X-ray analysis of the lectin-like domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1)" Acta Cryst. F F61, 524-527 (2005)

Ono,I., Mizuguchi,N., Nashima,N., Ono,N., and Tate,S. "A genetic algorithm for NMR three-dimensional protein structure determination by using Ninf-1 and Ninf-G" IPSJ Trans. on Advanced Computing System (ACS) 46, 396-406 (2005)

Nakashima,N., Matsubara,A., Ono,I., Ono,N., and Tate,S. "A genetic algorithm taking account of substructures for NMR three-dimensional protein structure determination" Proc. 2005 IEEE Congress on Evolutionary Computation 1761-1768 (2005)

Kasai,N., Tsunaka,Y., Ohki,I., Hirose,S, Morikawa,K., and Tate,S. "Solution structure of the HMG-box domain in the SSRP1 subunit of FACT" J.Biomol.NMR 32, 83-88 (2005).

Masuyama,M., Ishino,S., Nishino,T., Moriuchi,H.,Ueno,H., Morikawa,K., and Tate,S. "Resonance assignments for the DNA binding domain of ERCC-1/XPF heterodimer" J.Biomol.NMR 32, 175-175 (2005)

Tsunaka,Y., Kajimura,N., Tate,S. and Morikawa,K. "Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle" Nucleic Acids Res. 33, 3424-3434 (2005)