PITCh designer 2.0

A free online tool to design sgRNA and microhomologies for gene knock-in.

Citation information

PITCh designer (previous version : version 1.0):

PITCh systems:

Visit the old version of PITCh designer: version 1.0
*We do not recommend using Internet Explorer to use PITCh designer 2.0.

1. Enter nucleotide sequence

*PITCh system using Cpf1 is unconfirmed

Tm value
CG %
Primers for genotyping :